Mar. 2018
平成29年SUMSグランド・ラウンド優秀者表彰

Dec. 2017
第34回滋賀医科大学シンポジウム 若鮎賞(最優秀賞)

Pcgf5 controls the exit from totipotency in mouse embryonic stem cells.
Satoshi Mashiko; Shinnosuke Honda; Shuntaro Ikeda; Tomoyuki Tsukiyama
Biochemical and biophysical research communications, 11 Aug. 2026, Refereed

Reversible cystogenesis in juvenile primate ADPKD models: evidence from PKD1 heterozygous monkeys.
Shoma Matsumoto; Toshifumi Morimura; Kenichi Kobayashi; Keiichi Tsuji; Masataka Nakaya; Tomoyuki Tsukiyama; Ikuo Kawamoto; Takahiro Nakagawa; Etsuko Morishige; Koji Fukuda; Teppei Iwakiri; Kazuhiko Nozaki; Akihiro Kawauchi; Susumu Kageyama; Shinji Kume; Saori Nishio; Yasushi Itoh; Masatsugu Ema
Human molecular genetics, 23 Feb. 2026, Refereed

Evolutionarily divergent transcriptomic programs in ovarian folliculogenesis across mice, monkeys and humans.
Baku Nakakita; Ken Mizuta; Yoshitaka Katou; Hidetaka Tasaki; Tomonori Nakamura; Yukihiro Yabuta; Chizuru Iwatani; Hideaki Tsuchiya; Ikuo Kawamoto; Tomoyuki Tsukiyama; Xun Chen; Guillaume Bourque; Junko Otsuki; Mikiya Nakatsuka; Akihito Horie; Masaki Mandai; Mitinori Saitou; Hiroshi Ohta
Development (Cambridge, England), 01 Feb. 2026, Refereed

Non-viral generation of transgenic non-human primates via the piggyBac transposon system.
Masataka Nakaya; Chizuru Iwatani; Setsuko Tsukiyama-Fujii; Ai Mieda; Shoko Tarumoto; Taro Tsujimura; Takuya Yamamoto; Takafumi Ichikawa; Tomonori Nakamura; Ichiro Terakado; Ikuo Kawamoto; Takahiro Nakagawa; Iori Itagaki; Mitinori Saitou; Hideaki Tsuchiya; Tomoyuki Tsukiyama
Nature communications, 24 Mar. 2025, Refereed

Coupling of cell shape, matrix and tissue dynamics ensures embryonic patterning robustness.
Prachiti Moghe; Roman Belousov; Takafumi Ichikawa; Chizuru Iwatani; Tomoyuki Tsukiyama; Anna Erzberger; Takashi Hiiragi
Nature cell biology, Mar. 2025, Refereed

Temporal variability and cell mechanics control robustness in mammalian embryogenesis.
Dimitri Fabrèges; Bernat Corominas-Murtra; Prachiti Moghe; Alison Kickuth; Takafumi Ichikawa; Chizuru Iwatani; Tomoyuki Tsukiyama; Nathalie Daniel; Julie Gering; Anniek Stokkermans; Adrian Wolny; Anna Kreshuk; Véronique Duranthon; Virginie Uhlmann; Edouard Hannezo; Takashi Hiiragi
Science (New York, N.Y.), 11 Oct. 2024, Refereed

Expression pattern of Runt-related transcription factor (RUNX) family members and the role of RUNX1 during kidney development.
Keiko Yano-Sakamoto; Yuichiro Kitai; Naoya Toriu; Shinya Yamamoto; Ken Mizuta; Mitinori Saitou; Tomoyuki Tsukiyama; Ichiro Taniuchi; Motomi Osato; Motoko Yanagita
Biochemical and biophysical research communications, 30 Aug. 2024, Refereed

Atlas of Cynomolgus Macaque Hematopoiesis
Shinichiro Oshima; Rahul Sinha; Kazuhiro Takeuchi; Ken Mizuta; Tomonori Nakamura; Ryoji Ito; Ikuo Kawamoto; Chizuru Iwatani; Masataka Nakaya; Hideaki Tsuchiya; Shruti Bhagat; Tomoyuki Tsukiyama; Akifumi Takaori-Kondo; Mitinori Saitou; Yasuhiro Murakawa; Ryo Yamamoto
26 Apr. 2024, URL

Null mutation of exocyst complex component 3-like does not affect vascular development in mice.
Satsuki Takashima; Eiichi Okamura; Yusuke Ichiyama; Kiyoto Nishi; Akio Shimizu; Chisato Watanabe; Masanaga Muto; Shoma Matsumoto; Setsuko Tsukiyama-Fujii; Tomoyuki Tsukiyama; Hisakazu Ogita; Eiichiro Nishi; Masahito Ohji; Fumihiro Sugiyama; Satoru Takahashi; Seiya Mizuno; Ken-Ichi Mizutani; Masatsugu Ema
Experimental animals, 14 Feb. 2024, Refereed

Generation of a familial hypercholesterolemia model in non-human primate.
Akira Sato; Tomoyuki Tsukiyama; Masahiro Komeno; Chizuru Iwatani; Hideaki Tsuchiya; Ikuo Kawamoto; Mitsuru Murase; Takahiro Nakagawa; Iori Itagaki; Yasunari Seita; Shoma Matsumoto; Masataka Nakaya; Akio Shimizu; Atsushi Yamada; Masatsugu Ema; Hisakazu Ogita
Scientific reports, 20 Sep. 2023, Refereed

Apical-driven cell sorting optimised for tissue geometry ensures robust patterning
Prachiti Moghe; Roman Belousov; Takafumi Ichikawa; Chizuru Iwatani; Tomoyuki Tsukiyama; François Graner; Anna Erzberger; Takashi Hiiragi
17 May 2023

Induction of fetal meiotic oocytes from embryonic stem cells in cynomolgus monkeys.
Sayuri Gyobu-Motani; Yukihiro Yabuta; Ken Mizuta; Yoshitaka Katou; Ikuhiro Okamoto; Masanori Kawasaki; Ayaka Kitamura; Tomoyuki Tsukiyama; Chizuru Iwatani; Hideaki Tsuchiya; Taro Tsujimura; Takuya Yamamoto; Tomonori Nakamura; Mitinori Saitou
The EMBO journal, 02 May 2023, Refereed

Essential Roles of Exocyst Complex Component 3-like 2 on Cardiovascular Development in Mice.
Chisato Watanabe; Hirotoshi Shibuya; Yusuke Ichiyama; Eiichi Okamura; Setsuko Tsukiyama-Fujii; Tomoyuki Tsukiyama; Shoma Matsumoto; Jun Matsushita; Takuya Azami; Yoshiaki Kubota; Masahito Ohji; Fumihiro Sugiyama; Satoru Takahashi; Seiya Mizuno; Masaru Tamura; Ken-Ichi Mizutani; Masatsugu Ema
Life (Basel, Switzerland), 28 Oct. 2022, Refereed

Conformational change of RNA-helicase DHX30 by ALS/FTD-linked FUS induces mitochondrial dysfunction and cytosolic aggregates.
Ryota Hikiami; Toshifumi Morimura; Takashi Ayaki; Tomoyuki Tsukiyama; Naoko Morimura; Makiko Kusui; Hideki Wada; Sumio Minamiyama; Akemi Shodai; Megumi Asada-Utsugi; Shin-Ichi Muramatsu; Takatoshi Ueki; Ryosuke Takahashi; Makoto Urushitani
Scientific reports, 26 Sep. 2022, Refereed

Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeys.
Ken Mizuta; Yoshitaka Katou; Baku Nakakita; Aoi Kishine; Yoshiaki Nosaka; Saki Saito; Chizuru Iwatani; Hideaki Tsuchiya; Ikuo Kawamoto; Masataka Nakaya; Tomoyuki Tsukiyama; Masahiro Nagano; Yoji Kojima; Tomonori Nakamura; Yukihiro Yabuta; Akihito Horie; Masaki Mandai; Hiroshi Ohta; Mitinori Saitou
The EMBO journal, 15 Sep. 2022, Refereed

NPHP1ノックアウトカニクイザルの作成による若年性ネフロン癆の病態再現
鳥生 直哉; 中家 雅隆; 山田 龍; 好川 貴久; 岩谷 千鶴; 土屋 英明; 築山 勢津子[藤井]; 中川 孝博; 築山 智之; 柳田 素子
日本腎臓学会誌, May 2022

Chromosomal-scale de novo genome assemblies of Cynomolgus Macaque and Common Marmoset.
Vasanthan Jayakumar; Osamu Nishimura; Mitsutaka Kadota; Naoki Hirose; Hiromi Sano; Yasuhiro Murakawa; Yumiko Yamamoto; Masataka Nakaya; Tomoyuki Tsukiyama; Yasunari Seita; Shinichiro Nakamura; Jun Kawai; Erika Sasaki; Masatsugu Ema; Shigehiro Kuraku; Hideya Kawaji; Yasubumi Sakakibara
Scientific data, 28 Jun. 2021, Refereed

A MONKEY PKD1 MUTANT RECAPITULATES HUMAN AUTOSOMAL DOMINANT POLYCYSTIC KIDNEY DISEASE
小林憲市; 小林憲市; 築山智之; 中家雅隆; 依馬正次; 河内明宏
日本小児泌尿器科学会雑誌, Jun. 2021, URL

Non-human primates as a model for human development.
Tomonori Nakamura; Kohei Fujiwara; Mitinori Saitou; Tomoyuki Tsukiyama
Stem cell reports, 11 May 2021, Refereed

GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program.
Yoji Kojima; Chika Yamashiro; Yusuke Murase; Yukihiro Yabuta; Ikuhiro Okamoto; Chizuru Iwatani; Hideaki Tsuchiya; Masataka Nakaya; Tomoyuki Tsukiyama; Tomonori Nakamura; Takuya Yamamoto; Mitinori Saitou
Life science alliance, May 2021, Refereed

Induction of the germ cell fate from pluripotent stem cells in cynomolgus monkeys†.
Yoshitake Sakai; Tomonori Nakamura; Ikuhiro Okamoto; Sayuri Gyobu-Motani; Hiroshi Ohta; Yukihiro Yabuta; Tomoyuki Tsukiyama; Chiduru Iwatani; Hideaki Tsuchiya; Masatsugu Ema; Asuka Morizane; Jun Takahashi; Takuya Yamamoto; Mitinori Saitou
Biology of reproduction, 13 Mar. 2020, Refereed, Not invited

Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein.
Yasunari Seita; Toshifumi Morimura; Naoki Watanabe; Chizuru Iwatani; Hideaki Tsuchiya; Shinichiro Nakamura; Toshiharu Suzuki; Daijiro Yanagisawa; Tomoyuki Tsukiyama; Masataka Nakaya; Eiichi Okamura; Masanaga Muto; Masatsugu Ema; Masaki Nishimura; Ikuo Tooyama
Journal of Alzheimer's disease : JAD, 2020, Refereed, Not invited

Monkeys mutant for PKD1 recapitulate human autosomal dominant polycystic kidney disease.
Tomoyuki Tsukiyama; Kenichi Kobayashi; Masataka Nakaya; Chizuru Iwatani; Yasunari Seita; Hideaki Tsuchiya; Jun Matsushita; Kahoru Kitajima; Ikuo Kawamoto; Takahiro Nakagawa; Koji Fukuda; Teppei Iwakiri; Hiroyuki Izumi; Iori Itagaki; Shinji Kume; Hiroshi Maegawa; Ryuichi Nishinakamura; Saori Nishio; Shinichiro Nakamura; Akihiro Kawauchi; Masatsugu Ema
Nature communications, 11 Dec. 2019, Refereed, Not invited

Primate DNA/RNA database; development and application for medicine
KAWAI Jun; HIROSE Naoki; SANO Hiromi; OKAZAKI Yasushi; YAGI Ken; YAMAMOTO Yumiko; EMA Masatsugu; SEITA Yasunari; TSUKIYAMA Tomoyuki; NAKAYA Masataka; SASAKI Erika; INOUE Takashi; WATANABE Toshiaki; SAKAKIBARA Yasubumi; JAYAKUMAR Vasanthan; NAITO Yuki; MURAKAWA Yasuhiro; KAWAJI Hideya
Annual Meeting of the Japanese Society of Toxicology, Jun. 2019

Comprehensive evaluation of ubiquitous promoters suitable for the generation of transgenic cynomolgus monkeys†.
Yasunari Seita; Tomoyuki Tsukiyama; Takuya Azami; Kenichi Kobayashi; Chizuru Iwatani; Hideaki Tsuchiya; Masataka Nakaya; Hideyuki Tanabe; Seiji Hitoshi; Hiroyuki Miyoshi; Shinichiro Nakamura; Akihiro Kawauchi; Masatsugu Ema
Biology of reproduction, 01 Jun. 2019, Refereed, Not invited

Generation of an OCT3/4 reporter cynomolgus monkey ES cell line using CRISPR/Cas9.
Kenichi Kobayashi; Tomoyuki Tsukiyama; Masataka Nakaya; Susumu Kageyama; Keiji Tomita; Ryosuke Murai; Tetsuya Yoshida; Mitsuhiro Narita; Akihiro Kawauchi; Masatsugu Ema
Stem cell research, May 2019, Refereed, Not invited

Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells.
Chisato Watanabe; Jun Matsushita; Takuya Azami; Setsuko Tsukiyama-Fujii; Tomoyuki Tsukiyama; Seiya Mizuno; Satoru Takahashi; Masatsugu Ema
PloS one, 2019, Refereed, Not invited

Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis.
Arata Honda; Narantsog Choijookhuu; Haruna Izu; Yoshihiro Kawano; Mizuho Inokuchi; Kimiko Honsho; Ah-Reum Lee; Hiroki Nabekura; Hiroshi Ohta; Tomoyuki Tsukiyama; Yasuhide Ohinata; Asato Kuroiwa; Yoshitaka Hishikawa; Mitinori Saitou; Takamichi Jogahara; Chihiro Koshimoto
Science advances, May 2017, Refereed, Not invited

Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body.
Yasunari Seita; Tomoyuki Tsukiyama; Chizuru Iwatani; Hideaki Tsuchiya; Jun Matsushita; Takuya Azami; Junko Okahara; Shinichiro Nakamura; Yoshitaka Hayashi; Seiji Hitoshi; Yasushi Itoh; Takeshi Imamura; Masaki Nishimura; Ikuo Tooyama; Hiroyuki Miyoshi; Mitinori Saitou; Kazumasa Ogasawara; Erika Sasaki; Masatsugu Ema
Scientific reports, 25 Apr. 2016, Refereed, Not invited

Derivation of Induced Trophoblast Cell Lines in Cattle by Doxycycline-Inducible piggyBac Vectors.
Takamasa Kawaguchi; Dooseon Cho; Masafumi Hayashi; Tomoyuki Tsukiyama; Koji Kimura; Shuichi Matsuyama; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
PloS one, 2016, Refereed, Not invited

Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells.
Le Tran Phuc Khoa; Takuya Azami; Tomoyuki Tsukiyama; Jun Matsushita; Setsuko Tsukiyama-Fujii; Satoru Takahashi; Masatsugu Ema
PloS one, 2016, Refereed, Not invited

Correction: Establishment of trophoblast stem cells under defined culture conditions in mice
Ohinata, Y.; Tsukiyama, T.
PLoS ONE, 2015, Refereed, Not invited

Generation of Naïve Bovine Induced Pluripotent Stem Cells Using PiggyBac Transposition of Doxycycline-Inducible Transcription Factors.
Takamasa Kawaguchi; Tomoyuki Tsukiyama; Koji Kimura; Shuichi Matsuyama; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
PloS one, 2015, Refereed, Not invited

A hyperactive piggyBac transposon system is an easy-to-implement method for introducing foreign genes into mouse preimplantation embryos.
Shinnosuke Suzuki; Tomoyuki Tsukiyama; Takehito Kaneko; Hiroshi Imai; Naojiro Minami
The Journal of reproduction and development, 2015, Refereed, Not invited

Correction: A Comprehensive system for generation and evaluation of induced pluripotent stem cells using piggyBac transposition (PLoS ONE (2014) 9, 3 (e92973) DOI: 10.1371/journal.pone.0092973)
Tsukiyama, T.; Kato-Itoh, M.; Nakauchi, H.; Ohinata, Y.
PLoS ONE, 2014, URL

A comprehensive system for generation and evaluation of induced pluripotent stem cells using piggyBac transposition.
Tomoyuki Tsukiyama; Megumi Kato-Itoh; Hiromitsu Nakauchi; Yasuhide Ohinata
PloS one, 2014, Refereed, Not invited, Lead

A modified EpiSC culture condition containing a GSK3 inhibitor can support germline-competent pluripotency in mice.
Tomoyuki Tsukiyama; Yasuhide Ohinata
PloS one, 2014, Refereed, Not invited, Lead

Establishment of trophoblast stem cells under defined culture conditions in mice.
Yasuhide Ohinata; Tomoyuki Tsukiyama
PloS one, 2014, Refereed, Not invited, Last

Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system.
Tomoyuki Tsukiyama; Ryota Asano; Takamasa Kawaguchi; Narae Kim; Masayasu Yamada; Naojiro Minami; Yasuhide Ohinata; Hiroshi Imai
Genes to cells : devoted to molecular & cellular mechanisms, Jul. 2011, Refereed, Not invited, Lead

Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos.
Kei Miyamoto; Kouhei Nagai; Naoya Kitamura; Tomoaki Nishikawa; Haruka Ikegami; Nguyen T Binh; Satoshi Tsukamoto; Mai Matsumoto; Tomoyuki Tsukiyama; Naojiro Minami; Masayasu Yamada; Hiroyoshi Ariga; Masashi Miyake; Tatsuo Kawarasaki; Kazuya Matsumoto; Hiroshi Imai
Proceedings of the National Academy of Sciences of the United States of America, 26 Apr. 2011, Refereed, Not invited

Development of a simple and efficient method for generation of iPS cells by using piggyBac system to screen novel culture conditions
Tsukiyama, T.
Seikagaku, 2011, Refereed, Not invited

Cell-free extracts from mammalian oocytes partially induce nuclear reprogramming in somatic cells.
Kei Miyamoto; Tomoyuki Tsukiyama; Yang Yang; Ning Li; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
Biology of reproduction, May 2009, Refereed, Not invited

Reversible membrane permeabilization of mammalian cells treated with digitonin and its use for inducing nuclear reprogramming by Xenopus egg extracts.
Kei Miyamoto; Teruyoshi Yamashita; Tomoyuki Tsukiyama; Naoya Kitamura; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
Cloning and stem cells, Dec. 2008, Refereed, Not invited

Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes.
Toshiaki Watanabe; Atsushi Takeda; Tomoyuki Tsukiyama; Kazuyuki Mise; Tetsuro Okuno; Hiroyuki Sasaki; Naojiro Minami; Hiroshi Imai
Genes & development, 01 Jul. 2006, Refereed, Not invited

Roles of H2B E3 Ubiquitin ligase, RNF20 in gliomagenesis
DAUN Kenny; MORIMURA Naoko; TANIGAKI Kenji; TSUKIYAMA Tomoyuki; HAYASHI Yoshitaka; KOYAMA Natsu; SHIINO Akihiko; FUKAMI Tadateru; NOZAKI Kazuhiko; GO Yasuhiro; HITOSHI Seiji
日本生物学的精神医学会(Web), 2024

piggyBacトランスポゾンシステムを介した非ウイルス性トランスジェニックカニクイザルの作出
中家雅隆; 築山智之
日本分子生物学会年会プログラム・要旨集(Web), 2024

非ヒト霊長類におけるCD34陽性造血幹前駆細胞の分子的および機能的解析
大嶋 慎一郎; シンハ・ラフール; バーガット・シュルティ; 竹内 一博; 築山 智之; 伊藤 亮治; 村川 泰裕; 山本 玲
日本血液学会学術集会, Oct. 2023

NPHP1ノックアウトカニクイザルの作成による若年性ネフロン癆の病態再現
鳥生 直哉; 中家 雅隆; 山田 龍; 好川 貴久; 築山 智之; 柳田 素子; 伊佐 正
日本内科学会雑誌, Feb. 2023

Molecular and functional characterization of non-human primate hematopoiesis
OSHIMA Shinichiro; OSHIMA Shinichiro; SINHA Rahul; BHAGAT Shruti; TAKEUCHI Kazuhiro; TSUKIYAMA Tomoyuki; ITO Ryoji; MURAKAWA Yasuhiro; YAMAMOTO Ryo
日本血液学会学術集会抄録(Web), 2023

Exoc3l4 is essential for placental angiogenesis in mice.
高島さつき; 武藤真長; 渡部千里; 岡村永一; 松本翔馬; 築山智之; 藤井勢津子; 久保田慶顕; 水野聖哉; 杉山文博; 高橋智; 水谷健一; 依馬正次
日本分子生物学会年会プログラム・要旨集(Web), 2023

NPHP1ノックアウトカニクイザルの作成による若年性ネフロン癆の病態再現
鳥生直哉; 中家雅隆; 山田龍; 好川貴久; 岩谷千鶴; 土屋英明; 築山(藤井)勢津子; 中川孝博; 築山智之; 柳田素子
日本腎臓学会誌(Web), 2022

A MONKEY PKD1 MUTANT RECAPITULATES HUMAN AUTOSOMAL DOMINANT POLYCYSTIC KIDNEY DISEASE
小林憲市; 小林憲市; 築山智之; 中家雅隆; 依馬正次; 河内明宏
日本小児泌尿器科学会雑誌, 2021
https://search.jamas.or.jp/default/link?pub_year=2021&ichushi_jid=J02877&link_issn=&doc_id=20210624400004&doc_link_id=%2Fdw5syohi%2F2021%2F003001%2F005%2F0026-0036%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fdw5syohi%2F2021%2F003001%2F005%2F0026-0036%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

小児泌尿器科疾患:成育医療へのリレー 基礎研究が切り開く成育医療の未来
小林 憲市; 築山 智之; 影山 進; 依馬 正次; 河内 明宏
日本泌尿器科学会総会, Apr. 2019

Safety evaluations of intrabody against pathogenic TDP-43 aggregates in Macaca fascicularis
TAMAKI Yoshitaka; HIKIAMI Ryota; HIKIAMI Ryota; MINAMIYAMA Sumio; MINAMIYAMA Sumio; WADA Hideki; SHODAI Akemi; TSUKIYAMA Tomoyuki; MURAMATSU Shin-ichi; EMA Masatsugu; URUSHITANI Makoto
日本神経学会学術大会プログラム・抄録集, 2019

血管内皮細胞に高発現するエキソサイトーシス関連分子が担う新たな血管新生機構
渡部千里; 一山悠介; 築山勢津子; 築山智之; 水野聖哉; 高橋智; 依馬正次
日本分子生物学会年会プログラム・要旨集(Web), 2019

Hereditary kidney disease modeling in cynomolgus monkeys using genome editing
NAKAYA Masataka; NAKAGAWA Takahiro; FUKUDA Koji; IWAKIRI Teppei; IZUMI Hiroyuki; ITAGAKI Iori; NAKAMURA Shinichiro; KAWAUCHI Akihiro; EMA Masatsugu; TSUKIYAMA Tomoyuki; KOBAYASHI Kenichi; IWATANI Chizuru; TSUCHIYA Hideaki; SEITA Yasunari; MATSUSHITA Jun; KITAJIMA Kahoru; KAWAMOTO Ikuo
The Journal of Reproduction and Development Supplement, 2019

Primate DNA/RNA database; development and application for medicine
KAWAI Jun; NAKAYA Masataka; SASAKI Erika; INOUE Takashi; WATANABE Toshiaki; SAKAKIBARA Yasubumi; JAYAKUMAR Vasanthan; NAITO Yuki; MURAKAWA Yasuhiro; KAWAJI Hideya; HIROSE Naoki; SANO Hiromi; OKAZAKI Yasushi; YAGI Ken; YAMAMOTO Yumiko; EMA Masatsugu; SEITA Yasunari; TSUKIYAMA Tomoyuki
Annual Meeting of the Japanese Society of Toxicology, 2019

PKD医学の最先端 CRISPR/Cas9を用いたADPKDモデルカニクイザルの作出と解析
依馬 正次; 築山 智之; 小林 憲市; 和泉 博之; 河内 明宏
日本腎臓学会誌, Aug. 2018

PKD医学の最先端 CRISPR/Cas9を用いたADPKDモデルカニクイザルの作出と解析
依馬 正次; 築山 智之; 小林 憲市; 和泉 博之; 河内 明宏
日本腎臓学会誌, Aug. 2018, Not refereed, Not invited

Vegfr3レポーターマウスの作製と発現解析
渡部千里; 松下淳; 浅見拓哉; 築山(藤井)勢津子; 築山智之; 水野聖哉; 高橋智; 依馬正次
日本分子生物学会年会プログラム・要旨集(Web), 2018

Vegfr3レポーターマウスの作製と発現解析
渡部千里; 松下淳; 浅見拓哉; 築山(藤井)勢津子; 築山智之; 水野聖哉; 高橋智; 依馬正次
日本血管生物医学会学術集会プログラム・抄録集, 2018

Effect of the promoter on GFP expression and development of promoter evaluation system in cynomolgus monkey
SEITA Yasunari; TSUKIYAMA Tomoyuki; AZAMI Takuya; IWATANI Chizuru; TSUCHIYA Hideaki; NAKAMURA Shinichiro; EMA Masatsugu
The Journal of Reproduction and Development Supplement, 2018

非ヒト霊長類のトランスクリプトーム解析
廣瀬直毅; 佐野浩美; 八木研; 岡崎康司; 渡部聡朗; 井上貴史; 佐々木えりか; 中家雅隆; 築山智之; 清田弥寿成; 依馬正次; 村川泰裕; 川路英哉; 川路英哉; 河合純
日本分子生物学会年会プログラム・要旨集(Web), 2018

CRISPR/Cas9を用いた疾患モデルカニクイザルの作出
築山 智之; 小林 憲市; 岩谷 千鶴; 土屋 英明; 清田 弥寿成; 松下 淳; 北嶋 郁; 河本 育士; 中川 孝博; 福田 浩司; 岩切 哲平; 和泉 博之; 板垣 伊織; 中村 紳一朗; 河内 明宏; 依馬 正次
生命科学系学会合同年次大会, Dec. 2017, Not refereed, Not invited

VEGFR3レポーターマウスの作成と発現解析
渡部千里; 松下淳; 浅見拓哉; 築山(藤井)勢津子; 築山智之; 高橋智; 依馬正次
日本心血管内分泌代謝学会学術総会プログラム及び抄録集, 2017

全身でGFPを発現するトランスジェニックカニクイザルの作製
清田弥寿成; 築山智之; 岩谷千鶴; 松下淳; 土屋英明; 佐々木えりか; 依馬正次
Journal of Reproduction and Development, 2016

Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body
SEITA Yasunari; TSUKIYAMA Tomonori; IWATANI Chizuru; MATSUSHITA Jun; TUCHIYA Hideaki; SASAKI Erika; EMA Masatsugu
The Journal of Reproduction and Development Supplement, 2016

Knock in of expression-amplifying pluripotent-stem-cell reporters using CRISPR/Cas9 into mouse and cynomolgus monkey pluripotent stem cells.
TSUKIYAMA Tomoyuki; NIWA Hitoshi; IWATANI Chizuru; TSUCHIYA Hideaki; SEITA Yasunari; MATSUSHITA Jun; EMA Masatsugu
The Journal of Reproduction and Development Supplement, 2015

piggyBac トランスホソンを用いたリフログラミンク因子導入によるウシ羊膜細胞からの栄養膜細胞株樹立の試み
川口 高正; 築山 智之; 松山 秀一; 木村 康二; 南 直治郎; 山田 雅保; 今井 裕
第107回日本繁殖生物学会大会, Aug. 2014, Not refereed, Not invited

piggyBacトランスポゾンを用いたリプログラミング因子導入による ウシ羊膜細胞からの栄養膜細胞株樹立の試み
川口 高正; 築山 智之; 松山 秀一; 木村 康二; 南 直治郎; 山田 雅保; 今井 裕
日本繁殖生物学会 講演要旨集, 2014

ヒトおよびカニクイザル多能性幹細胞の培養におけるWnt阻害剤の影響
築山 智之; 依馬 正次
日本繁殖生物学会 講演要旨集, 2014

蛍光レポーターによるPGC形成過程、精子形成過程、卵形成過程の可視化
大日向 康秀; 築山 智之
The Journal of Reproduction and Development, Aug. 2013

Generation of murine-ES-cell-like iPS cells in cattle using piggyBac transposition of doxycycline-inducible transcription factor
T. Kawaguchi; T. Tsukiyama; N. Minami; M. Yamada; K. Kimura; H. Imai
CiRA International Symposium 2013 ~Raising the next generation of stem cell research~, Kyoto, Japan, 11 Mar. 2013, Refereed, Not invited

piggyBacトランスポゾンを用いたナイーブ型ウシiPS細胞の樹立の試み
川口 高正; 築山 智之; 南 直治郎; 山田 雅保; 松山 秀一; 木村 康二; 今井 裕
日本繁殖生物学会 講演要旨集, 2013

哺乳動物における新型多能性幹細胞株の樹立
築山智之; 築山智之; 築山智之; 浅野良大; 今井裕; 丹羽仁史; 大日向康秀; 大日向康秀
J Reprod Dev, 20 Aug. 2012, Not refereed, Not invited

イヌにおけるマウスES細胞様のiPS細胞の誘導
浅野 良太; 川口 高正; 南 直治郎; 山田 雅保; 今井 裕; 築山 智之; 鳩谷 晋吾; 杉浦 喜久弥; 稲葉 俊夫
日本生殖医学会雑誌, Jul. 2012

Transdifferentiation of bovine aminion cells into trophoblast-like cells using piggyBac transposition of doxycycline-inducible reprogramming factors
T. Kawaguchi; T. Tsukiyama; N. Minami; M. Yamada; K. Kimura; H. Imai
Satellite symposium to the International Congress on Animal Reproduction (ICAR), Jul. 2012, Refereed, Not invited

Derivation of murine-ES-cell like iPS cells in canine under the EOS reporter system
T. Kawaguchi; R. Asano; Tsukiyama. T; N. Minami; M. Yamada; S. Hatoya; K. Sugiura; T. Inaba; H. Imai
International Society for Stem Cell Research (ISSCR), Jun. 2012, Refereed, Not invited

Establishment of induced pluripotent stem cells in cattle using piggyBac transposition of doxycycline-inducible transcription factors
T. Kawaguchi; T. Tsukiyama; K. Kimura; N. Minami; M. Yamada; H. Imai
World Congress on Reproductive Biology (WCRB), Cairns, Australia, 09 Oct. 2011, Not refereed, Not invited

piggyBacシステムを用いた簡便で効率的なiPS細胞誘導法の開発と培養条件評価系への応用
築山 智之
生化学, Sep. 2011
https://search.jamas.or.jp/default/link?pub_year=2011&ichushi_jid=J00725&link_issn=&doc_id=20111011320005&doc_link_id=1520853833409415808&url=https%3A%2F%2Fcir.nii.ac.jp%2Fcrid%2F1520853833409415808&type=CiNii&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00003_1.gif

イヌにおける人工多能性幹細胞樹立の試み
浅野良太; 築山智之; 南直治郎; 山田雅保; 今井裕; 鳩谷晋吾; 杉浦喜久弥; 稲葉俊夫
第136回日本生殖医学会関西支部集談会, 05 Mar. 2011, Refereed, Not invited

ウシにおける人工多能性幹細胞の樹立の試み
川口高正; 築山智之; 南直治郎; 山田雅保; 今井裕; 木村康二
日本生殖医学会雑誌, 2011

Induction of ground state pluripotency from mammalian somatic cells
TSUKIYAMA Tomoyuki; ASANO Ryota; IMAI Hiroshi; OHINATA Yasuhide
日本分子生物学会年会プログラム・要旨集(Web), 2011, Not refereed, Not invited

様々な動物種におけるマウスES細胞様・ヒトES細胞様iPS細胞樹立の試み
築山智之; 浅野良太; 南直治郎; 山田雅保; 今井 裕
第9回日本再生医療学会、講演要旨集 P-045、広島国際会議場, Mar. 2010, Not refereed, Not invited

Generation of porcine induced pluripotent stem cells
TSUKIYAMA Tomoyuki; ASANO Ryota; MINAMI Naojiro; YAMADA Masayasu; IMAI Hiroshi
The Journal of Reproduction and Development Supplement, 2009

Analysis of culture condition for generation of induced pluripotent stem (iPS) cells in various mammalian species
TSUKIYAMA Tomoyuki; ASANO Ryota; MINAMI Naojiro; YAMADA Masayasu; IMAI Hiroshi
日本分子生物学会年会講演要旨集, 2009, Refereed, Not invited

Mammalian oocyte extracts induce chromatin remodeling and dedifferentiation of somatic cells, but do not global demethylation
K. Miyamoto; T. Tsukiyama; N. Minami; M. Yamada; H. Imai
REPRODUCTION IN DOMESTIC ANIMALS, Jul. 2008, Refereed, Not invited

Histone Acetylation is Important for NANOG Expression in Reprogrammed Somatic Cells by Extracts from Porcine Oocytes at the Germinal Vesicle Stage
Miyamoto Kei; Tsukiyama Tomoyuki; Yang Yang; Li Ning; Minami Naojiro; Yamada Masayasu; Imai Hiroshi
The Journal of Reproduction and Development Supplement, 2008

Reprogramming of mouse embryonic fibroblasts by cell-free extracts from nanog-overexpressing embryonic stem cells
Tomoyuki Tsukiyama; Kei Miyamoto; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
BIOLOGY OF REPRODUCTION, 2008, Refereed, Not invited

DNA demethylation associated with nuclear reprogramming of the somatic cell genome in cell-free extracts from mammalian oocytes
Kei Miyamoto; Tomoyuki Tsukiyama; Yang Yang; Ning Li; Naojiro Minami; Masayasu Yamada; Hiroshi Imai
BIOLOGY OF REPRODUCTION, 2008, Refereed, Not invited

Nuclear reprogramming and dedifferentiation of somatic cells by cell-free system derived from porcine oocytes
Miyamoto Kei; Tsukiyama Tomoyuki; Minami Naojiro; Yamada Masayasu; Imai Hiroshi
The Journal of Reproduction and Development Supplement, 2007

Differential nuclear reprogramming of somatic cells by porcine germinal vesicle and metaphase II oocyte extracts.
Miyamoto K; Tsukiyama T; Minami N; Yamada M; Imai H
The 4th Asian Reproductive Biotechnology Conference, 2007, Refereed, Not invited

Two novel classes of small RNAs from mouse germline
Watanabe T; Takeda A; Tsukiyama T; Sasaki H; Minami N; Imai H
International Genomic Imprinting Workshop, 2006, Refereed, Not invited

マウス表現型解析スタンダード : 系統の選択、飼育環境、臓器・疾患別解析のフローチャートと実験例
伊川 正人; 高橋 智; 若菜 茂晴
羊土社, 2016, Not refereed
9784758101981

霊長類ゲノム工学開発コア (PRiME)における遺伝子改変カニクイザルの作出
築山 智之
関西共創の場 若手人材育成セミナー, 14 Oct. 2025, Invited

霊長類ゲノム工学開発コア (PRiME)における遺伝子改変カニクイザルの作出
築山 智之
京都大学ヒト行動進化研究センターセミナー, 01 Oct. 2025, Invited

遺伝子改変カニクイザルの作出
築山 智之
革新脳分科会The Final, Mar. 2024, Invited

遺伝子改変カニクイザルの作出
築山 智之
日本受精着床学会, Jul. 2023, Invited

Generation of transgenic cynomolgus monkeys using piggyBac transposition.
Tsukiyama T.
日本分子生物学会, Dec. 2021, Invited

Disease modeling in cynomolgus monkeys using CRISPR/Cas9.
Tsukiyama T.
日本発生生物学会, May 2020, Invited

CRISPR/Cas9を用いたカニクイザルにおける疾患モデリング
築山 智之
日本マーモセット研究会大会, Feb. 2020, Invited

CRISPR/Cas9を用いた遺伝性腎疾患モデルカニクイザルの作出
築山 智之; 小林 憲市; 中家 雅隆; 岩谷 千鶴; 土屋 英明; 清田 弥寿成; 松下 淳; 北嶋 郁; 河本 育士; 中川 孝博; 福田 浩司; 岩切 哲平; 和泉 博之; 板垣 伊織; 中村 紳一朗; 河内 明宏; 依馬 正次
日本分子生物学会, Nov. 2018, Invited

CRISPR/Cas9を用いた疾患モデルカニクイザルの作出
築山 智之
関西血管生物研究会, Oct. 2017, Invited

iPS細胞誘導技術を駆使した新型多能性幹細胞培養条件の探索
築山 智之
東京大学医科学研究所共同研究拠点事業 平成２４年度若手研究者シンポジウム, Nov. 2012, Invited

家畜ならびに大型実験動物における多能性幹細胞株の樹立と応用
築山 智之; 今井 裕
日本生化学会九州支部例会シンポジウム, May 2010, Invited

発生学
滋賀医科大学

THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

SOCIETY FOR REPRODUCTION AND DEVELOPMENT

ゲノム編集霊長類を用いた前頭葉機能とその障害の生成機構に関する研究開発
Aug. 2024, Mar. 2030

腎臓老化の責任細胞の同定と病態形成機構の解明
基盤研究(B)
京都大学
01 Apr. 2026, 31 Mar. 2029

Elucidation of the stem cell hierarchy that constitutes the placenta and functional improvement of trophoblast stem cells
Grant-in-Aid for Scientific Research (A)
Kyoto University
01 Apr. 2024, 31 Mar. 2029

霊⻑類と集合管オルガノイドを活⽤した若年性ネフロン癆の治療標的の同定
May 2026, Mar. 2029

内在性機構の惹起による革新的遺伝子ノックイン
挑戦的研究(萌芽)
滋賀医科大学
26 Jun. 2026, 31 Mar. 2028

モザイク性の解消による早期表現型解析のための遺伝子改変技術基盤の構築
基盤研究(B)
滋賀医科大学
01 Apr. 2025, 31 Mar. 2028

非ウイルス性トランスポゾンベクターを用いたトランスジェニックサルの作出
Jul. 2023, Mar. 2028

Elucidating the pathogenesis of and development of therapeutic strategies against the neuropsychiatric disorders in the primate models by utilizing the gene introduction and manipulation technologies
Grant-in-Aid for Scientific Research (S)
National Institute for Physiological Sciences
01 Apr. 2026, 31 Mar. 2027

Elucidating the pathogenesis of and development of therapeutic strategies against the neuropsychiatric disorders in the primate models by utilizing the gene introduction and manipulation technologies
Grant-in-Aid for Scientific Research (S)
Kyoto University
27 Apr. 2022, 31 Mar. 2027

能動的組換えによる革新的遺伝子ノックイン
挑戦的研究(萌芽)
滋賀医科大学
28 Jun. 2024, 31 Mar. 2026

Primate Nephrology: analyzing primate-specific repair mechanisms of renal injury
Grant-in-Aid for Scientific Research (B)
Kyoto University
01 Apr. 2024, 31 Mar. 2026

Primate Nephrology: analyzing primate-specific repair mechanisms of renal injury
Grant-in-Aid for Scientific Research (B)
Kyoto University
01 Apr. 2023, 31 Mar. 2026

Spatio-temporal variabilities and robustness in pre-implantation mammalian development
Grant-in-Aid for Scientific Research (S)
Kyoto University
05 Jul. 2021, 31 Mar. 2026

遺伝子改変動物作出におけるモザイク性の解消
基盤研究(B)
滋賀医科大学
01 Apr. 2024, 31 Mar. 2025

幹細胞を用いた羊膜前駆細胞の発生機序と細胞特性解析
基盤研究(B)
京都大学
01 Apr. 2024, 31 Mar. 2025

Partial reprogramming of trophoblast stem cells to improve placental function
Grant-in-Aid for Challenging Research (Exploratory)
Kyoto University
30 Jun. 2023, 31 Mar. 2025

遺伝子改変動物作出におけるモザイク性の解消
基盤研究(B)
滋賀医科大学
01 Apr. 2022, 31 Mar. 2025

幹細胞を用いた羊膜前駆細胞の発生機序と細胞特性解析
基盤研究(B)
京都大学
01 Apr. 2022, 31 Mar. 2025

Development of innovative knock-in technology by active gene transfer
Grant-in-Aid for Challenging Research (Exploratory)
Shiga University of Medical Science
30 Jun. 2022, 31 Mar. 2024

能動的遺伝子導入による革新的ノックイン技術の開発
Sep. 2022, Dec. 2023

非ウイルス性トランスポゾンベクターを用いたトランスジェニックサルの作出
Jul. 2020, May 2023

Establishment of a technology platform for genetic modification of fertilized eggs that enables analysis from the F0 generation
Grant-in-Aid for Transformative Research Areas (B)
Shiga University of Medical Science
02 Oct. 2020, 31 Mar. 2023

Cellular senescence of renal "subpopulations" influences renal aging and injury responsiveness
Grant-in-Aid for Scientific Research (B)
Kyoto University
01 Apr. 2020, 31 Mar. 2023

Development of innovative knock-in technology using endogenous homologous recombination mechanism
Grant-in-Aid for Challenging Research (Exploratory)
Shiga University of Medical Science
30 Jul. 2020, 31 Mar. 2022

Development of diagnosis and therapy for aging using a nonhuman primate model of premature aging
Grant-in-Aid for Scientific Research (C)
Shiga University of Medical Science
01 Apr. 2019, 31 Mar. 2022

Mechanism and Reconstitution In VItro of Human Germ Cell Development
Grant-in-Aid for Specially Promoted Research
Kyoto University
25 Apr. 2017, 31 Mar. 2022

Establishment of high-quality novel ES / iPS cells that precisely reflect the in vivo state
Grant-in-Aid for Scientific Research (C)
Shiga University of Medical Science
01 Apr. 2017, 31 Mar. 2020

Establishment of novel pluripotent stem cells using genetically modified cynomolgus ES and iPS cells.
Grant-in-Aid for Young Scientists (B)
Shiga University of Medical Science
01 Apr. 2015, 31 Mar. 2017

Establishment of novel pluripotent stem cells in mammals by iPS technology.
Grant-in-Aid for Young Scientists (B)
独立行政法人理化学研究所
01 Apr. 2013, 31 Mar. 2015

ES細胞由来タンパク質導入によるiPS細胞作製に関する研究
特別研究員奨励費
京都大学
2009, 2010

特開2019-216666, 特願2018-117705, 早期老化症モデル動物
伊藤 靖; 小笠原 一誠; 依馬 正次; 築山 智之; 土屋 英明; 石垣 宏仁